LTER Baixo Sabor: Long term monitoring of freshwater fish - Sabor watershed [2012 - 2020]

The data was collected between 2012 and 2020, using standard electrofishing protocols (INAG, 2008) in a stream stretch of 50 meters for 15-25 minutes, with longer surveys in wider and deeper streams to properly cover the stretch. Within each segment, fish were sampled using a single anode electrofishing gear (350-750V, 3-5A, DC). Electrofishing was always conducted by the same operator, while a second operator was tasked with the netting of the displaced fish. All captured individuals were identified on site and released after the sampling session. In accordance with the Portuguese legislation, all individuals belonging to exotic species were euthanized, instead of being returned to the wild. All sampling were conducted under the appropriate permits of Portuguese conservation authority, the Instituto da Conservação da Natureza e Florestas (ICNF). Details of the methods are available in Ferreira et al. (2016) and Mota-Ferreira et al. (2021).