The objective of long-term study at Lysina and Pluhův Bor is to compare biogeochemical patterns in forested catchments with contrasting lithologies that can serve as examples of distinct sensitivity to anthropogenic acidification. The catchments represent two geochemically contrasting, acidic (Podzols on granite) and ultrabasic (Stagnosols on serpentinite) sites, both covered with managed forests. The both catchments have full infrastructure for hydrological nad biogeochemical research: V-notch weirs, precipitation and throughfall collectors, lysimeters, piezometers, boreholes, etc. Vast number of data from the research is stored in the Czech Geol. Survey, Prague (P. Krám, site manager and the founder of the research sites), the Czech GEOMON database (F. Oulehle, Czech Geol. Surv.), the ICP Integrated Monitoring dtb. (S. Kleemola, Fin. Envir. Inst., Helsinki), the ICP Waters dtb. (Heleen de Wit, Norw. Inst. of Water Res., Oslo), and in the Critical Zone Exploration Network (N. Nikolaidis, Tech. Univ. of Crete, Chania).